Evaluation of antioxidant, anti-cancer and anti-inflammatory characteristics of bio-synthesized silver nanoparticles produced by waste extract of Rheum ribes L.

Background: This study was designed to evaluate the antioxidant, anti-inflammation and anti-cancer potency of silver nanoparticles produced by waste Rheum ribes L. extract. Materials and Methods: in this study, 10 gr of dried Rheum ribes L powder after 3 hours dissolving at 37°C was filtered and applied for silver nanoparticles preparation. Particle size, X-ray diffractometer (XRD), Fourier transform infrared radiometer (FT-IR) and transmission electron microscope (TEM) methods were used to measurement the properties of silver nanoparticles. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) method accompanied by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) compounds were used to evaluate the cytotoxicity and antioxidant capacity of the nanoparticles. Results: The size of the produced silver nanoparticles was 28 nm with a uniform distribution and spherical appearance. The effective antioxidant dose for the ABTS and DPPH compounds was 200 µg/ml, and the obtained IC50 on HDF, Huvec, and MCF-7 cells for 24, 48 and 72 hours treatment were 30, 25, 12; 13.2, 11, 10; and 10, 9 and 8 µg/ml, respectively. Conclusion: The silver nanoparticles produced by waste Rheum ribes L. extract can have potential for use as a therapeutic strategy against cancer.